Our SAR expertise was combined with our collaborators’ (MerlPham, Dey, Hauck, Vlot) proteomics expertise to provide the first and only comprehensive study of proteins that accumulate in the phloem during the plant immune response to disease. Label-free quantitative LC-MS/MS provided a comprehensive view of the immune response phloem sap proteome. Both novel and previously known proteins were identified and we demonstrated that a number of these phloem proteins is essential for the plant immune response.
Impact
Our novel and significant findings were published in the high impact journal Plant Physiology and we put these findings into perspective in an invited review in Frontiers in Plant Science. The data will serve as an excellent resource for plant immunity and phloem researchers for many years. Plant Physiology 2016, 171:1495-1510 Front.Plant Sci. 2016, 7:651.
Student Experience
This international collaboration included a week-long visit by myself and a month long visit by my Ph.D. student Phil Carella to Dr. Vlot’s lab to perform proteomics. This was an invaluable experience in which both plant immunity and proteomics expertise were available to me and my student, resulting in successful completion of this project which resulted in a high impact publication. Other students in my lab also benefited as they contributed to this project. The students in Dr. Vlot’s lab also benefited by interacting with both me and my student Phil.
Countries
Germany
Impact
Research, Global Partnerships
Institutional Partner(s)
Helmholtz Zentrum, German Research Centre of Environmental Health, Institute for Biochemical Plant Pathology, Munich Germany
Community Partner(s)
Industry Partner(s)
Key Outcomes
Publications
Sponsorship
Federal Foreign
Sponsorship Details
My NSERC Discovery funded the phloem exudate collection and plant immunity part of the collaboration, while German grants to Dr. Corina Vlot and Dr. Merl-Pham funded the proteomics work.
